Abstract:Phoebe bournei, one of the national second-class endangered plants, is the excellent precious timer species with broad market prospect. Leaf spot was newly found leaf disease, which resulted in stress and abnormal growth of plants in the extension of Ph. bournei plantation. The causing agent of the disease was identified as Pestalotiopsis microspora by morphological characteristics and rDNA-ITS sequences analysis. The result of the biological test showed that the optimal conditions for mycelia growth of Pe. microspora strain ZZ-03 were Czapek agar or PDA+PBL(pH 6.0)at 28℃ in dark. The spore production was optimal on PDA medium(pH 8.0)at 28℃ in dark. Conidia germination was positively correlated with relative humidity at 28℃ with pH 8.0 in dark. The best carbon and nitrogen sources for mycelia growth were glucose and beef extract, respectively, while glucose and peptone were the best for conidiation, respectively.
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