RNA-mediated virus resistance is an effective way to obtain virus resistant plants. The strategy is provided with the advantages of high bio-safety and long resistant duration. The method is regarded as a potential strategy with broad application value and practical significance in plant genetic engineering against viruses. In this study,we used untranslatable CP genes of Potato virus X(PVX-CP) and Potato virus Y(PVY-CP) to construct a chimeric cDNA fragment,which was then introduced into the plant expressing vector pROKⅡ. The recombinant binary vector pROKXY was introduced into tobacco (NC89) plants via Agrobacterium tumefaciens-mediated transformation,six transgenic plants immune to the co-infection with PVX and PVY^N were obtained. Molecular analysis demonstrated that the resistance was RNA-mediated virus resistance.