YANG Di, LI Yanjia, CAI Qichang, HUANG Jie, LI Ying, WANG Baotong, LONG Shusheng, LI Qiang
Stripe rust and powdery mildew are two important diseases on wheat worldwide, breeding and gro-wing resistant cultivars is the most economical and effective method to control these diseases. To determine the level of resistance and distribution of resistance genes in commercial wheat cultivars and regional trial lines to stripe rust and powdery mildew in stripe rust and powdery mildew main epidemic areas Shaanxi, Gansu, Sichuan, and Qinghai in China, 194 wheat cultivars (lines) were inoculated with the Chinese prevalent Puccinia striiformis f.sp. tritici (Pst) and Blumeria graminis f. sp. tritici (Bgt) races in both seedling and adult-plant stages, respectively. The results showed that 81 (41.80%) and 33 (17.00%) wheat entries were resistant to the mixture of Pst and Bgt races at the seedling stage, respectively; and 137 (70.60%) and 111 (57.20%) entries showed resistance to Pst and Bgt at the adult-plant stage, respectively. Also, the stripe rust and powdery mildew resistance genes were detected using Genotyping by Targeted Sequencing (GBTS) technology. The results indicated that 16, 9, 68, 62, 31, 88, 3, 5, 16, 9 and 1 entries were postulated to carry the Yr17, Yr26, Yr29, Yr30, Yr75, Yr78, Yr80, Yr82, YrAK58, YrSP and Pm21 genes, respectively; and 85, 30, 145, 47, 8, 78, and 89 entries may carry QYrsn.nwafu-1BL, QYrsn.nwafu-2AS, QYrqin.nwafu-2AL, QYrhm.nwafu-2BC, QYrsn.nwafu-3DL, QYr.nwafu-4BL, QYrsn.nwafu-6BS stripe rust QTL loci, respectively. No entries were detected to carry Yr18, Pm2a, Pm5e, and PmV resistance genes. This study provides a theoretical basis for breeding wheat with disease-resistant genes and distributing them rationally.