Genes for resistance to powdery mildew in six new wheat lines were analyzed. Susceptible variety Liaochun10 was crossed with SM20121, SM203390, SM20125, SM200332, SM20126 and SM20005 respectively, and hemi-diallel cross combination was established with six resistant new lines. The seedlings of parents, F₁, F₂ and F₃ family lines from the cross combinations were inoculated with race No. 15 of Blumeria graminis f. sp. tritici. Inheritance analysis showed that the six lines contained one dominant gene for resis-(tance) to race No. 15 of B. g. f. sp. tritici respectively. Allelic analysis concluded that four lines, SM20121, SM203390, SM20125 and SM200332, contained the gene Pm12, SM20126 contained the gene Pm21 and SM20005 contained the gene Pm16 resistant to powdery mildew. The six wheat lines were proposed as excellent resistant parents.