Physio-biochemical characterization and molecular identification of <i>Burkholderia cepacia</i> isolate Lyc2 as a PGPR to cotton seedlings

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Acta Phytopathologica Sinica ›› 2007, Vol. 37 ›› Issue (4) : 426-432.

Physio-biochemical characterization and molecular identification of Burkholderia cepacia isolate Lyc2 as a PGPR to cotton seedlings

YU Xiao-qing¹, XI Li-jun¹, LIU Yong-guang¹, LI Guang-wei^1,3, LAN Yu-fei¹, ZHU Xiao-ping¹, LI Xiang-dong^1,2

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Abstract

A bacterial strain, Lyc2, was isolated from tobacco rhizosphere and showed remarkable inhibition to the mycelial growth of multiple pathogenic fungi in dual culture. In greenhouse, strain Lyc2 showed 48.8% efficiency to control cotton seedling damping-off. In solution culture experiment, compared with control, Lyc2 significantly increased the fresh weight and dry weight of cotton seedlings. The morphological, physiological and biochemical characteristics of Lyc2 were similar to those of Burkholderia cepacia. Homology analysis of 16S rDNA and ITS sequences showed that Lyc2 was closely related to B. cepacia. Further results of recA gene sequence comparison showed that Lyc2 belonged to B. pyrrocinia, genomovar IX of B. cepacia complex.

Key words

PGPR / Burkholderia cepacia / 16S rDNA / 16S-23S rDNA internally transcribed spacer(ITS) / recA gene

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YU Xiao-qing, XI Li-jun, LIU Yong-guang, LI Guang-wei, LAN Yu-fei, ZHU Xiao-ping, LI Xiang-dong. Physio-biochemical characterization and molecular identification of Burkholderia cepacia isolate Lyc2 as a PGPR to cotton seedlings[J]. Acta Phytopathologica Sinica, 2007, 37(4): 426-432