The full length cDNA of S10 sequence of Rice gall dwarf virus (RGDV) Guangdong isolate was cloned into pMD18-T vector and the complete nucleotide sequence was determined. The results showed that S10 had a length of 1198 nt encoding a polypeptide of 320 amino acids with a Mr of 36 kDa. Sequence comparison showed that the S10 of RGDV Guangdong isolate had the same genome organization with the Thailand isolate, and shared 96.2% nucleotide and 98.8% amino acid sequence identities. The predicted polypeptide of RGDV $10 also shared 29% and 33% identities respectively with the proteins encoded by Rice dwarf virus (RDV) S9 and Wound tumor virus (WTV) S11. The RGDV S10 cDNA was cloned to pET28b (+) and highly expressed in Escherichia coli BL21 (DE3), and the expressed recombinant protein was purified by His. Bind column. These works will promote the structural and functional research of RGDV S10 protein.