Cleome witches'-broom phytoplasma(CWB) collected from Hainan Province,was detected and identified using DAPI fluorescence microscopy,PCR,clone and sequencing techniques.Three different type universal primer pairs of phytoplasma were used to amplify 1.43 kb fragment of 16S rDNA,0.36 kb fragment of 16S-23S rDNA and 1.3 kb fragment of rp DNA.Then PCR-amplified products were cloned and sequenced.The results of sequencing and homologuous comparison with other phytoplasmas showed that cleome witches'-broom phytoplasma shared 99.9% similarity with peanut witches'-broom phytoplasma in 16S rRNA gene,100% in 16S-23S rRNA gene and 99.7% in rp gene.Cleome witches'-broom phytoplasma might be sorted into peanut witches'-broom group(16SrⅡ),16SrⅡ-A according to the results of RFLP and sequencing of 16S rDNA.