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Acta Phytopathologica Sinica 1997 Vol.27
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THE DEFINITE MEANING OF THE CHINESE TRANSLATION OF THE TERM FUNGI
Qiu Weifan
Acta Phytopathologica Sinica 1997, 27 (
1
): 1-2.
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The English term fungus (pl. fungi) should be translated into Chinese in two ways, according to its definite meaning when applied to different cases. One is pronounced
zhenjun
(真菌) for true fungi which is of strict sense, usually for those belonging to Eumycota while the other is of broad sense, pronounced
junwu
(菌物) for organisms including true fungi, pseudofungi, fungus like protozoa and symbiontic fungi such as lichens and mycorrhyza. So far the term fungi in the 8th edition (1995) of Ainsworth & Bisby's DICTIONART OF FUNGI is of broad sense, therefore the Chinese term
junwu
(菌物) should be used.
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THE KINGDOMS OF FUNGI
Xu Tong, Ge Qixin
Acta Phytopathologica Sinica 1997, 27 (
1
): 3-4.
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THE BREAKDOWN OF RESISTANCE TO STRIPE RUST IN FAN 6-MIAN YANG WHEAT CULTIVARS AND STRATEGIES FOR ITSCONTROL
Niu Yongchun, Wu Liren
Acta Phytopathologica Sinica 1997, 27 (
1
): 5-8.
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115
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A PRELIMINARY STUDY ON THE DIFFERENTIATION OF THE RESISTANCE IN WHEAT CULTIVAR TO STRIPE RUST (
PUCCINIA STRIIFORMIS
)
Jing Jinxue, Shang Hongsheng, Li Zhengqi, Wang Meinang
Acta Phytopathologica Sinica 1997, 27 (
1
): 9-16.
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ifferentiation of the resistance in wheat cultivars to stripe rust (
Puccinia striiformis
) was tested with wild type physiological race CYR29-1 and its 7 mutants. The results indicated that the populations of Suwon 11, Kang yin 655, and Beardless Zhong 4 differentiated in their resistance to these stripe race and mutants. So were some sources of resistance, commercial cultivars and candidate cultivars, with different ratios of R:S or R:MR individuals. All these indicates that the populations of these accessions are heterogeneous in their resistance to stripe rust. The cause of the differentiation of the resistance of wheat cultivars to stripe rust was also discussed, and the improvement on the screening and utilization of sources of resistance suggested.
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THE RESISTANCE REACTION OF
H. VILLOSA
TO POWDERY MILDEW ISOLATES AND ITS EXPRESSION IN WHEAT BACKGROUND
Chen Xiao, Shi Ainong, Shang Liming, Leath Steven, Murphy J. Paul
Acta Phytopathologica Sinica 1997, 27 (
1
): 17-22.
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Resistance to eighty four isolates of
Blumeria graminis
f. sp.
tritici
was identified in
Haynaldia villosa
(2n=14, VV), five amphiploids (2n=42, AABBVV), TH1, TH1W, TH2W, TH3, and TH3W, derived from crossing
Triticum durum
(2n=28,AABB) with
H.villosa
, and hexaploid substitute wheat lines 6D/6V, pm93064 (DH
2
), derived from
H. villoas
. The results showed that the
H. villosa
and its derived wheat lines were immune or high resistant to all tested isolates from America, Germany and China. The resistance was more effective than any other resistance gene lines from pm1 to pm20, and the resistance gene has been transfered effectively to tetraploid and hexaploid wheat from
H. villosa
, and also was inherited to the progenies.
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THE FUZZY CLUSTERING ANALYSIS OF WHEAT SHARP EYESPOT'S FIELD EPIDEMIC DYNAMICS
Shi Mingwang, Xu Ruofu, Ru Zhenggang, Liu Mingtao, Zhao Qixue
Acta Phytopathologica Sinica 1997, 27 (
1
): 23-27.
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This paper analyses the field epiemic dynamics of wheat sharp eyespot, using the method of Fuzzy clustering. The results show:the field epidemic dynamics of the disease can be divided into 5~6 stages, namely the occurrence stage before winter, winter steady stage, turning green rising stage, jointing evil stage and white ear showing illness stage. The field distribution and expanding of wheat sharp eyespot can be described with several parameters, such as X1, X2, X3, X4. Fuzzy clustering analysis describes the condition of the field distribution of wheat sharp eyespot, of both horizontal expanding and vertical directions. That is, it describes the changing of the disease in time and space. The results provide the reliable basis for forecast and integrated control of wheat sharp eyespot.
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IDEN TIFICATION OF RESISTANCE OF SOYBEAN GERMPLASM FROM NORTH CHINA TO SOYBEAN MOSAIC VIRUS
Yang Chongliang, Shang Youfen, Li Changsong, Zhao Jiuhua, Xin Xiangqi, Luo Ruiwu
Acta Phytopathologica Sinica 1997, 27 (
1
): 27-28.
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56
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ON THE TECHNIQUE OF ISOLATION AND THE PATHOGENICITY OF THE MAIZE STALK ROT PATHOGENS
Wu Quanan, Zhu Xiaoyang, Lin Hongxu, Jin Jiatong, Wang Guiyiang
Acta Phytopathologica Sinica 1997, 27 (
1
): 29-35.
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In 1985~1995, 1394 samples of maize stalk rot were isolated from 16 provinces in China. of which, 63.8% were Pythium, 36.4% were Fusarirm. Isolation frequency of these fungi were, however, different among these provinces. Results of further research showed that the frequency were directly influenced by several factors, such as culture medium, isolation position, degree of stalk rot, as well as season when the sample was collected.
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DETECTION OF MAIZE DWARF MOSAIC VIRUS IN MAIZE SEED
Ma Zhanhong, Li Huaifang, Qiu Weifan
Acta Phytopathologica Sinica 1997, 27 (
1
): 36-36.
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126
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MECHANISM OF RESISTANCE TO RACE 4 OF
HETERODERA GLYCINES
IN CHINESE BLACK SOYBEANS Ⅱ. ULTRASTRUCTURE OF SYNCYTIA IN THE ROOTS OF RESISTANT AND SUSCEPTIBLE SOYBEANS
Yan Qingshang, Chen Pinsan, Wang Lianzheng
Acta Phytopathologica Sinica 1997, 27 (
1
): 37-41.
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The present paper deals with studies on the ultrastructure of syncytia in roots of resistant soybean varieties, Huipizhiheidou and Yuanboheidou,and susceptible control, Ludou No.1 at 4, 9, 14 days after inoculation. The results showed that there were bigger syncytium component cells, formation of all ingrowths and a lot of various organelles, especially with more and bigger smooth endoplasmic reticula in susceptible roots; whereas the syncytia in resistant roots were with smaller syncytium component cells, and there were more ribosomes, and less and smaller rough endoplasmic reticula,and more lipidlike droplets in their cytoplasm.It was also observed that the separation of plasmalmma from cell wall and the early degeneration of cytoplasm in resistant roots also occurred.
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ON THE CAUSAL FUNGUS OF WAMPEE SHOOT ROT
Zhang Chuanfei, Qi Peikun
Acta Phytopathologica Sinica 1997, 27 (
1
): 42-42.
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167
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THE RATIO OF CYTOKININ TO AUXIN IN RELATION TO THE OCCURENCE OF SWEET POTATO WITCHES' BROOM SYMPTOM
He Fangting, Wu Hongjin, Chen Ziwen, Dai Qun
Acta Phytopathologica Sinica 1997, 27 (
1
): 43-46.
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The variations of cytokinin and auxin levels in the sweet potato associated with MLO were measured by using HPLC in the whole growing season. It was indicated that the cytokinin level of diseased sweet potato was much higher than that of healthy ones, and as to the auxin level, the diseased plants had drasticly lower concentration of auxin than healthy plants in the bud bursting stage. The auxin level in both healthy and diseased plants became almost the same as the sweet potato growing up. The cytokinin to auxin ratio during the growing season was calculated and itsèffect on the witches' broom symptoms was discussed.
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EFFECTS OF SOME ECOLOGICAL FACTORS ON THE SCLEROTIAL ROT OF
CONIOTHYRIUM MINITANS
Jiang Daohong, Li Guoqing, Yi Xianhong, Wang Daoben
Acta Phytopathologica Sinica 1997, 27 (
1
): 47-52.
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The sensitivities of
Sclerotinia
spp. isolates from different hosts infected by
Coniothyrium minitans
were tested in laboratory. The effects of soil factors,such as soil temperature,soil related water content and soil type on
C. minitans
parasitizing sclerotia of
Sclerotinia sclerotiorum
were examined. The activity and colonization of
C. minitans
in soil were also detected by measuring the respiratory rate of soil when
C. minitans
was inoculated in laboratory. The results showed that the sclerotia of all
Sclerotinia
spp. isolates tested were high sensitive to
C. minitans
rather than to
S. minor
which showed moderate sensitivity. The optimum soil temperatrue and soil related water content (RWC) for parasitism were 20℃ and 50~60% RWC respectively. The sclerotia carried spores of
C. minitans
were rotten after two months in all kinds of soil so far supplied,but the soil type could influence the speed of sclerotial rot.The activity and colonization of
C. minitans
in soil were related closely with the existence of sclerotia or extract of sclerotia of S. sclerotiorum. According to the ecological characteristics of
C. minitans
, it suggested that
C. minitans
has a great potential for controlling sclerotinia wilt of various crops.
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BIOLOGY AND SEROLOGICAL COMPARISON OF TOMV TOMATO ISOLATE AND TMV BROAD BEAN ISOLATE AND SPECIFIC DETECTION WITH POLYMERASE CHAIN REACTION
Zhou Xueping, Xue Chaoyang, Liu Yong, Li Debao
Acta Phytopathologica Sinica 1997, 27 (
1
): 53-58.
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Biology and serological comparison between ToMV tomato isolate (ToMV-To-S1) and TMV broad bean isolate (TMV-B935A) was made. Both ToMV-To-S1 and TMV-B935A could infected 14 plant species in 3 families when 20 plant species in 6 families were tested. The difference was found in
Nicotiana tabacum
(
N. tabacum
-White Burley,
N. tabacum
-Huang Miaoyu), ToMV-To-S1 induced local lesions in these plant species, while TMV-B935A produced systemic mosaic symptoms. In double-diffusion tests, spur formation was observed between ToMV-To-S1 and TMV-B935A, and the results of intragel cross-absorption tests agreed with the doublediffusion tests results. Specific primers corresponding to the coat protein gene and 3' non coding region were designed. When ToMV-To-S1 cDNA templete were used, a specific band could be amplified with ToMV primers by PCR, No band was found when we used TMV primers. With TMV-B935A templete, only TMV primers could produce a specific band. So it is easy to distinguish ToMV-To-S1 and TMV-B935A with ToMV and TMV specific primers.
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A METHOD FOR DETERMINATION OF RESISTANCE TO TOMATO
FUSARIUM
WILT IN GREENHOUSE
Bai Minzhan, Zheng Guibin
Acta Phytopathologica Sinica 1997, 27 (
1
): 59-63.
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The results of this study indicated that the optimal stage of tomato seedling inoculated by root dipping for resistance identification to
Fusarum oxysporum
is at 2-true-leaf stage, when the fibrous roots are numerous and liable to be hurt. Thus resulted in high inoculation rate by root dipping. The occurrence of
Fusarium
wilt showed no significant difference when inoculated plants were re-planted at once or at 2, 5, 10 minutes after dipping. With an inoculum concentration of 10 spores per milliliter and at 28℃~30℃ in a greenhouse,resistance of tomato varieties to Fusarium oxysporum can be determined and screened accurately.
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IDENTIFICATION OF LR13 BY MEANS OF HYBRID NECROSIS NE2
Guo Aiguo, Zhang Fengguo, Zhu Zhiyu
Acta Phytopathologica Sinica 1997, 27 (
1
): 64-64.
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105
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RELATIONSHIP BETWEEN SUGAR,LIGNIN CONTENT AND RESISTANCE TO DOWNY MILDEW OF CUCUMBER
Luo Guifen, Cui Juntao, Gao Yufang, Zhang Li
Acta Phytopathologica Sinica 1997, 27 (
1
): 65-69.
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The experiment utilized two induced nonpathogenic fungi to inject in the stem of cucumber plant. The results shown that the active resistance and against ability to downy mildew were improved. All the foliages sugar and lignin content of the induced plants were higher than the contrast plant. The sugar were 11.21%, 10.66% and 9.58%, respectively. The lignin were 8.4%, 7.8% and 6.9%, respectively. These data indicated the induced-resistance caused by induced fungi related to the contents of soluble sugar and lignin, but also demonstrated that the contents of soluble sugar and lignin in cucumber plants were a part of mechanism of inducing resistance.
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IDENTIFICATION OF THE PATHOGEN OF GUAVA TRUNK BLIGHT
Liu Ren, Liang Guangsheng, Qi Peikun
Acta Phytopathologica Sinica 1997, 27 (
1
): 70-70.
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147
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INSPECTION ON THE BROWN ROT OF
SPATHIPHYLLUM PALLAS
Jiang Zide, Qi Peikun
Acta Phytopathologica Sinica 1997, 27 (
1
): 71-74.
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Spathiphyllum pallas
Hort.is a new introduced flower plant in South China in recent years. The brown rot occured seriously in the autunm of 1995. The pathogen has been identified as
Cylindrocladium spathiphylli
Schoulties et al. according to its morphology, cultural characters and pathogenicity. A series of experiments on diseased soil and various media of planting flower plants showed that this fungus spread to Mainland from Taiwan province with the seedlings and their media in 1995. It is proposed that roots of plant and media must be quarantined strictly during introducing these new plants.
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INDEXING OF SEEDS OF GOUTOUCHENG SOUR ORANGE FOR CITRUS TRISTEZA-STEM PITTING VIRUS
Chen Guoqing, Wang Hongxiang, Yan Senxiang
Acta Phytopathologica Sinica 1997, 27 (
1
): 75-78.
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Indexing tests were done in seeds of Guotoucheng orange for citrus tristeza-stem pitting virus with double antibody sandwish enzyme-linked immunosorbent assay (DAS-ELISA). The results showed that seeds collected from Guotoucheng orange seedling infected with stem pitting disease were positive for CTV-SP. The consistency of CTV in inner seed-coat was higher than that in peeled seed. It remained positive after one to two years' storage of seeds in refrigerator at 4℃, but the consistency of CTV was reduced. External seed-coat was negative. Young tissues collected from some of the seedlings propagated from infected seeds, as well as Mexican lime grafted on Guotoucheng orange seedlings were positive for CTV by DAS-ELISA test. More than 100 of 2 years-old Guotoucheng orange seedlings planted in insect-proof screenhouse were grafted with buds of healthy Mexican lime, and no symtoms were observed for two years.
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EFFECT OF BANANA BUNCHY TOP VIRUS (BBTV) ON ENDOGENOUS HORMONES OF BANANA PLANT
Zhang Haibao, Zhu Xiru, Liu Hongxian
Acta Phytopathologica Sinica 1997, 27 (
1
): 79-83.
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107
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Three endogenous hormone contents of banana plants infected by BBTV, including gibberellic acid (GAs), isopentenyladenine group (iPAs) and abscisic acid (ABA), were detected by enzyme-linked immunosorbent assay (ELISA) in this test. The results showed that the GAs contents of inoculated plants at different infecting periods were all lower than that of healthy plants, in spite of increasing slightly during infection. The iPAs contents of infected plants decreased significantly after 14th day of BBTV inoculation and kept in lower level during infection. ABA was greatly induced and accumulated in banana plants infected by BBTV. The ABA content of infected plants tested in 35th day of BBTV inoculation was highest and was 3.34 times of the control. The movement of BBTV in infected banana plants was determined in the same time in this test. The BBTV contents in inoculated leaves and in top leaves detected by indirect-ELISA respectively indicated that BBTV particles replicated greatly both in inoculated leaves and top leaves after 21th day of inoculation. However, the symptom of infected banana plants appeared in top leaves after 35th day of inoculation. All these results indicated that the symptom of banana bunchy top disease may be closely related to the unbalance of endogenous hormones of infected plants, but indirectly related to the BBTV movement within plant.
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PHENOLICS AND PEROXIDASES DURING COTTON RESISTANT TO
XANTHOMON AS CAMPESTRIS
PV.
MALVACEARUM
(RACE 18)
Nicole, M., Dai G. H., Geiger J. P., Martinez, C., Andary, C., Bresson E., Daniel, J.
Acta Phytopathologica Sinica 1997, 27 (
1
): 84-84.
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94
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ON THE COMBINED INFECTION OF ROOT ROT PATHOGENS ON
PANAX NOTOGINSENG
Luo Wenfu, Yu Shengfu, He Chenfu, Li Zhongyi, Wang Chaoliang, Cui Xiuming
Acta Phytopathologica Sinica 1997, 27 (
1
): 85-91.
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Pseudomonas
sp.,
Fusarium solani, Alternaria tenuis
and
Rhabditis elegans
were isolated from root rot of
Panax notoginseng
(Sanqi) at different developmental stages. In inoculation test,
Pseudomonas
sp. showed the strongest pathogenicity, while
Fusarium solani
and
Alternaria tenuis
were weak,
Rhabditis elegans
was non-pathogenic. With the three formers as inocula, the root rot rates of mixed inoculations were all higher than that of their individual inoculations. The combined inoculation of the three pathogens showed the highest pathogenicity. With new multiple range test, root rot rates among 3 combined inoculations and their individual inoculation resulted in significant diffenence (P=0.05). The present experiments reveals the fact that the bacteria played an important role in the combination infection with other fungous and nematode pathogens.
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NEW STRATEGY FOR TRANSGENE MEDIATED RESISTANCE TO TOBACCO MOSAIC VIRUS
Sun Fengcheng
Acta Phytopathologica Sinica 1997, 27 (
1
): 92-92.
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78
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CLONING AND EXPRESSION OF 75KDA READTHROUGH PROTEIN GENE AND ITS 54KDA FRAGMENT FROM BEET NECROTIC YELLOW VEIN VIRUS
Li Dawei
Acta Phytopathologica Sinica 1997, 27 (
1
): 92-93.
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64
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IDENTIFICATION, PURIFICATION AND CHARACTERIZATION OF RICE LIPOXGENASES INDUCED IN RESPONSE TO INFECTION OF RICE BLAST FUNGUS
Qin Yinping
Acta Phytopathologica Sinica 1997, 27 (
1
): 93-94.
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74
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POTENTIAL VIRUS-VECTOR NEMATODES ASSOCIATED WITH FRUIT TREES IN CHINA AND THEIR TRANSMISSION OF CERTAIN VIRUSES
Wang Shouhua
Acta Phytopathologica Sinica 1997, 27 (
1
): 94-95.
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96
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DEFECTIVE MUTANTS OF
PSEUDOMONAS SOLANACEARUM
IN EXTRACELLULAR PROTEINS AND THEIR PATHOGENESIS
Kang Yaowei
Acta Phytopathologica Sinica 1997, 27 (
1
): 95-95.
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90
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ISOLATION, PURIFICATION, CHARACTERIZATION AND IMMUNOGOLD LOCALIZATION
IN VIVO
OF AN ANTI-BACTERIAL PROTEIN FROM THE POTATO
Yuan Fenghua
Acta Phytopathologica Sinica 1997, 27 (
1
): 95-96.
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67
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MOLECULAR BIOLOGY OF PLANT DISEASE RESISTANCE
Zhang Deshui, Chen Shouyi
Acta Phytopathologica Sinica 1997, 27 (
2
): 97-103.
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102
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DISCUSSION ABOUT THE CHINESE TRANSLATION OF TECHNICAL TERMS IN PLANT PATHOLOGY AND ITS RELATED SCIENCES
Qiu Weifan
Acta Phytopathologica Sinica 1997, 27 (
2
): 104-106.
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The present papaer deals with the accurate meaning of the Chinese translation of some technical terms in plantpathology and its related sciences. The writer inter prets some of the published Chinese terms with their accurate meanings and points out the proper selection in use of them. The terms mentioned and discussed are as the following:fungi, virus, virion, particle, toxin, mechanism, principle, infection, infection cycle, life cycle, nucleoside, mycoplasma, mycoplasma-like organisms, phytoplasma, bacte-ria-like organisms, antagonism, syner-gism and Cynophyta or green bacteria, and nucleocapsid.
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STUDY ON WILD RELATIVES OF WHEAT FOR RESISTANCE TO HEAD SCAB
Wan Yongfang, Yan Ji, Yang Junliang, Liu Faquan
Acta Phytopathologica Sinica 1997, 27 (
2
): 107-111.
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276 accessions from 80 species of 16 genera in Triticeae were screened for resistance to head scab with single-floret and multi-floret injection inoculation methods respectively. The results showed that great difference in resistance existed between genera or species.
Roegneria
species with high resistance to initial infection and high resistance to pathogen spread were the most resistant ones in all tested species.
Elytrigia, Pseudoroegeria, Psathyrostachys
and perennial species of
Hordeum
were susceptible to initial infection, but were highly resistant to pathogen spread.
Elymus, Kengyilia
and
Agropyron
showed moderate resistance to initial infection and high resistance to pathogen spread.
Aegilops, Crithopsis, Eremopyrum, Heteranthelium, Henrardia, Haynaldia, Teaniatherum
and annual wild species of
Hordeum
showed high susceptibility to head scab.
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IDENTIFICATION OF TOBACCO MOSAIC VIRUS INFECTING SWEET POTATO
Xin Xiangqi, Li Changsong, Yang Chongliang, Shang Youfen, Zhao Jiuhua
Acta Phytopathologica Sinica 1997, 27 (
2
): 112-112.
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THE RELATIONSHIP BETWEEN ASCORBIC ACID AND RESISTANCE OF WHEAT TO SCAB
Chen Lifeng, Ye Maobing, Chen Yongxing, Xu Langlai, Xu Yonggao
Acta Phytopathologica Sinica 1997, 27 (
2
): 113-118.
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Four winter wheat varieties were inoculated with conidia suspension of
Fusarium graminearum
Schw. JF-12, and conc. of ascorbic acid (AsA) and the activities of AsA oxidase and AsA peroxidase in the head tissues sampled 2~22 d after inoculation were assayed. The results showed that susceptible and moderately susceptible varieties (Ning No.6, Yangmai No.3 and Ning 8026) showed higher conc. of AsA and lower activities of AsA oxidase and AsA peroxidase, while resistant variety (Wangshuibai) showed lower conc. of AsA and higher activities of AsA oxidase and AsA peroxidase in the inoculated heads than those in the check heads (inoculated with water). It was also observed that AsA stimulated the germination of conidia and the extension of the germ tubes of the pathogenic fungus at the conc. of 1~5mg AsA·10ml
-1
. AsA showed no obvious effects on the growth of the pathogen at the conc. of ≤ 15mg·L
-1
but suppressed the fungal growth at the conc. of 30~60 mg·L
-1
. The conc. of AsA on which the fungus grown gave no effect on the conc. of AsA within the fungal mycelia. The relationship between wheat resistance/susceptibility to scab and AsA conc. in the heads tissues was discussed and it was suggested that higher AsA contents might be related to wheat susceptibility to scab.
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MECHANISMS OF IMMUNIZATION OF RICE SEEDLINGS
Yu Yunzhen, Liu Jianhua, Wang Junli, Cao Yuanyin, Yang Jiashu
Acta Phytopathologica Sinica 1997, 27 (
2
): 119-124.
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The rice seedling disease causal agents:
Fusarium equiseti, F. oxysporum, F. graminearum
and
Rhizoctonia solani
isolated from paddy rice soil and diseased rice seedlings, were used to inoculate the Immunization of Rice Nursery (IRN) seedlings and Paddy Soil Land Nursery (PSLN) seedlings (control). It was indicated that the disease resistance of IRN seedlings was 95.5~97.8% higher than that of PSLN seedlings. By testing the dynamics defensive enzyme systems in both IRN and PSLN seedlings after inoculation with the causal agents, the results showed that under the elicitment by the causal agents the enzyme activity peaks of peroxidase (PO) and superoxide dismutase (SOD) in IRN seedlings appeared 36 hrs and 4 hrs earlier than those in PSLN seedlings, respectively, and that the ratio activity of phinylalaminase in IRN seedlings was 2 times more than that in PSLN seedlings. In addition, IRN seedlings metabolized faster and showed higher physiological activity. The physiological traits of IRN seedlings were as follows:(1) the higher amount of chlorophyll (3.12mg/ml), strong photosynthetic ability (887.0 mol O
2
/g.hr), thus dry matter accumulation was much faster. (2) Dense root system (11.5 roots per plant), there was 3.9 roots per plant more than that of control. (3) The α Naphthylamine oxidation was 60.0
μ
g/g.hr which was 30% higher than that of control.
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GROWTH AND MORPHOGENESIS OF LEAF CULTURE IN VITRO FROM SOYBEAN PLANTS INOCULATED WITH SOYBEAN MOSAIC VIRUS (SMV)
Guo Zibiao, Gai Junyi
Acta Phytopathologica Sinica 1997, 27 (
2
): 125-128.
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78
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Callus induction and bud and root differentiation of leaf culture
in vitro
from soybean plant inoculated with soybean mosaic virus strains Sa and Sg were studied. The induction rate and its results showed that there appeared no significant difference of callus morphological characteristics between those inoculated and non-inoculated with SMV. The frequency of bud differentiation for those inoculated with Sa strain was lower than those non-inoculated, while the situation was
vise versa
for those of Sg. The root differentiation ability was decreased after inoculation of Sa and Sg, but with less influence on resistant varieties in comparison with susceptible varietes.
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PRELIMINARY STUDY ON DIFFERENTIATION OF PATHOGENICITY OF
FUSARIUM SOLANI
CAUSING SOYBEAN ROOT ROT
Li Changsong, Zhao Jiuhua, Yang Chongliang, Shang Youfen, Luo Reiwu, Xin Xiangqi, Xing Han, Zhao Jingrong
Acta Phytopathologica Sinica 1997, 27 (
2
): 129-132.
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96
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Soybean cultivars and lines 861387-10, Ludou No 4, 8205, RN-9, 861003, Peking were selected as differential hosts for pathogenicity tests. 37 isolates of soybean root rot pathogen (
Fusarium solani
) collected from Shandong, Anhui, Henan and Jingsu provinces were divided into 10 groups based on the virulence on 6 differentials.
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THE ANTIBIOTIC ACTION OF ZHONGSHENGMYCIN
Jiang Xiliang, Xie Deling, Ni Chufang, Zhu Changxiong, Song Peiguo
Acta Phytopathologica Sinica 1997, 27 (
2
): 133-138.
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433
)
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The inhibiting action of ZHONGSHENGMYCIN on gram postive bacteria, gram negative bacteria and filamentous fungi showed that ZHONGSHENGMYCIN was a wide-spectrum agricultural antibiotics. It had no effect on permeability of cell membrane of bacteria, but it could result in condensation of the plasma of some cells of
E. carotovora
subsp.
carotovora
on high concentration 100 ppm. The incorporation experiment of precursor labelled by radio-active isotope showed that ZHONGSHENGMYCIN had little effect on the synthesis of DNA and RNA of
E. carotovora
subsp.
carotovora
on 15.6 ppm, while it had strong inhibiting action on the synthesis of protein. Similar results to precursor incorporation experiment was gained by quantity analysis the amount of the macromolecules of
Bacillus subtilis
6633. The studies showed the mode of action of ZHONGSHENGMYCIN is the inhibiting action on the synthesis of protein.
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USING THE BIOLOG IDENTIFICATION SYSTEM FOR RAPID IDENTIFICATION OF BEAN WILT BACTERIUM (
CURTOBACTERIUM FLACCUM FACIENS
PV.
FLACCUM FACIENS
)
Zhao Youfu, Wei Yadong, Gao Chongsheng, Zhao Longzhang, Huang Guoming, Huang Qinglin
Acta Phytopathologica Sinica 1997, 27 (
2
): 139-144.
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79
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The Biolog identification system (Biolog Inc. USA, BIS) with MicroStation
TM
V3.5 software were used for the rapid identification of bean wilt bacterium (
C. f
. pv.
flaccumfaciens
, Cff) and related plant coryneform bacteria. The results showed that 23 of 24 isolates of Cff and related pathovars (pv.) were correctly identified to species level, one to genus level; and 13 out of 24 isolates to pv. level with an accuracy of 95.8% being identified to species level. Two isolates of
Clavibacter michiganensis
subsp.
michiganensis
and two isolates of
C.m
. subsp.
insidiosus
were also correctly identified to pv.level. Cluster analysis using MLClust program showed that Cff and related coryneform bacteria isolates seperated into two divisions and supported the classification of
Clavibacter
and
Curtobacterium
at both species and genus level.We concluded that the BIS is a useful tool for the rapid identification of Cff and meets the requirements of our port plant quarantine because of its rapid, accurate and high standards.
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THE STRUCTURAL SENESCENCE OF APPLE BARK IN RELATION TO THE RESISTANCE TO
VALSA CERATOSPERMA
Wang Jinyou, Li Meina, Qi Yongan, Wang Chuntian
Acta Phytopathologica Sinica 1997, 27 (
2
): 145-148.
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79
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An invastigation on the relation between bark senescing process and susceptibility to
Valsa
invasion was conducted. The number of circles of phloem fiber bundles is corresponding to the age of apple, bark growth. During the senescing process of bark, the disturbance and disorganization of the end part of phloem rays in the outer old bark tissues is closely related to susceptible performance of the bark. Characteristic standard of "strong tree" was suggested and verified in field trials. Artificial renewal of senescent bark was helpful for enhancing resistance to fungal invasion and therefore prevention of disease incidence.
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