PLANT DISEASE AND CONTROL
HE Tao, DU Hongyan, SUN Jiaxiu, CHEN Jiyue, DENG weiping, ZHU Shusheng, HE Xiahong, DU Fei
In order to investigate the changes of microbial community in the leaves of different grape after the application of dimethomorph, the field ‘red earth’ grape was used as the research object, and the pesticide dimethomorph was applied continuously to collect samples from different groups, with the changes of the community structure of the fungi and bacteria in the grape leaf analyzed by high-throughput sequencing technology using Illumina HiSeq. Based on the analysis of species classification of operational taxonomic units (OTUs), a total of 6 phylum, 23 classes, 60 orders, 131 families, 212 genera and 296 species were obtained. The bacterial community has 42 phylum, 91 classes, 222 orders, 398 families, 846 genera and 1 469 species.
Conclusion: In the ‘red earth’ grape base cultivated in sandy soil and hedgerow of Yunnan Agricultural University, it was found that: (1) The dominant fungi among the grape leaves were Cladosporium, Erysiph, unclassified-k_- Fungi, symmetrospora; The dominant bacteria were Lactobacillius, Bifidobacterium, Bacteroides, Enterococcus, unclassified-f-lachnospiraceae, norank-f-muribaculaceae, Bacillus, Blautia and Kosakonia. (2) After treatment with dimethomorph, the abundance and diversity of the leaf Fungi Community decreased slightly, the diversity increased significantly, the abundance and diversity of the bacterial community increased, but the richness and diversity of the leaf fungi and bacteria community decreased significantly in the sporadic leaves. (3) Among the leaves of sporadic grape, the difference between the leaf Fungi Community and the bacterial community in the leaves of grape was decreased. (4) In healthy and diseased leaves, the fungi with higher relative abundance were Erysiph, Mycospharella, Filobasidium, Nigrospora, Gibberella, Mortierella and Fusarium, and were the bacteria Streptococcus, Staphylococcus and Campylobacter were the most common pathogens; Cladosporium, Septoria, Alternaria and Erythrobasidium were the fungi with lower relative abundance; The bacteria were Kosakonia and Sphingomonas; It is concluded that these bacteria were sensitive to dimethomorph. (5) The effect of dimethomorph pesticide on the microorganism in grape leaves was different between healthy leaves and sporadic leaves, and new dominant bacteria appeared.
