海南长春花黄化病植原体的16S rDNA序列分析研究

车海彦, 罗大全, 符瑞益, 叶莎冰, 吴云锋

植物病理学报 ›› 2009, Vol. 39 ›› Issue (2) : 212-216.

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PDF(284 KB)
植物病理学报 ›› 2009, Vol. 39 ›› Issue (2) : 212-216.
研究简报

海南长春花黄化病植原体的16S rDNA序列分析研究

  • 车海彦1,2, 罗大全2, 符瑞益2, 叶莎冰2, 吴云锋1
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Sequence analysis of 16S ribosomal DNA of phytoplasma associated with periwinkle yellows disease in Hainan

  • CHE Hai-yan1,2, LUO Da-quan2, FU Rui-yi2, YE Sha-bing2, Wu Yun-feng1
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摘要

Periwinkle(Catharanthus roseus) yellows is a common disease in Hainan. Periwinkle's leaf tissue with symptoms was assayed for phytoplasma infection by using PCR assay employing phytoplasma universal 16S rRNA gene primers (Rl6mF2/Rl6mR1). A PCR product (about 1.4 kb) was amplified from periwinkle showed yellows. Nucleotide sequencing and phylogenetic tree analysis showed that the amplified 16S rDNA contained 1 432 nucleotides, the most homology was 98.1% with the members of elm yellows group (16S r Ⅴ) and clustered in the same clade, while it was under 96.1% with other phytoplasma groups. Our results suggested that the phytoplasma sample belonged to 16S rⅤgroup and was tentatively named as Hainan periwinkle yellows phytoplasma (PY-Hn). This is the first report of existence of 16S r Ⅴ group phytoplasma in naturally infected periwinkle.

Abstract

Periwinkle(Catharanthus roseus) yellows is a common disease in Hainan. Periwinkle's leaf tissue with symptoms was assayed for phytoplasma infection by using PCR assay employing phytoplasma universal 16S rRNA gene primers (Rl6mF2/Rl6mR1). A PCR product (about 1.4 kb) was amplified from periwinkle showed yellows. Nucleotide sequencing and phylogenetic tree analysis showed that the amplified 16S rDNA contained 1 432 nucleotides, the most homology was 98.1% with the members of elm yellows group (16S r Ⅴ) and clustered in the same clade, while it was under 96.1% with other phytoplasma groups. Our results suggested that the phytoplasma sample belonged to 16S rⅤgroup and was tentatively named as Hainan periwinkle yellows phytoplasma (PY-Hn). This is the first report of existence of 16S r Ⅴ group phytoplasma in naturally infected periwinkle.

关键词

periwinkle yellows disease / Phytoplasma / 16S rDNA / sequence analysis

Key words

periwinkle yellows disease / Phytoplasma / 16S rDNA / sequence analysis

引用本文

导出引用
车海彦, 罗大全, 符瑞益, 叶莎冰, 吴云锋. 海南长春花黄化病植原体的16S rDNA序列分析研究[J]. 植物病理学报, 2009, 39(2): 212-216
CHE Hai-yan, LUO Da-quan, FU Rui-yi, YE Sha-bing, Wu Yun-feng. Sequence analysis of 16S ribosomal DNA of phytoplasma associated with periwinkle yellows disease in Hainan[J]. Acta Phytopathologica Sinica, 2009, 39(2): 212-216

基金

国家自然科学基金资助项目(30660103)
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