本文是报道一种简易的方法诱发Gibberella zeae的子囊壳,以鉴定禾谷镰刀菌的菌株。即:在泥钵内装灭菌的砂或(石至)石,其上种植纯菌株培养的带菌麦粒,然后在钵面复盖消毒纱布,并保持砂和纱布的潮湿。在20℃和装置20瓦黑光灯为光源照射的温箱中,3~6天形成子囊壳,10~12天发育成熟的子囊孢子,提供镜检鉴定。在15~25℃的自然散射光下,供试的481个禾谷镰刀菌菌株,获同样结果。同时,选用其他四种镰刀菌(即燕麦、锐顶、串珠、雪腐镰刀菌)和禾谷镰刀菌的代表菌株,进行交错污染试验,在限定的观察时间内,未见有相互干扰。
禾谷镰刀菌寄主广,所致麦、玉米上的病害损失大。本研究所提出的方法可以解决该菌在PSA培养基上大型分生孢子不易产生或产生亦形态多变所造成鉴定上的困难,且在病害测报和防治研究上有其应用价值。

This Paper describes a method of developing perithecial stage of Gibberella zeae.Perithecia were readily produced from mycelial culture in the tubes of autoclaved wheat grains when a few hyphae-covered grains of wheat were placed on the surface of autoclaved moist sand or vermi-culite in pots.Each pot was covered on the top with a moistened cheese cloth.