The fuzzy or delinted cotton seeds,preheating in 80℃ water for 2 minutes,were soaked in Czapek's Liquid medium in which the spores of Fusarium or Verticillium were mixed.After 24 hours at 25℃(by constant shaking at a rate of 55-60/min.),97.6% seeds were inoculated with the pathogen on the outside while 23.2% inside.The inoculated seeds can be thoroughly disinfected safely by soaking in a solution of BCM(F.W.) 0.3%(a.i) room temperature for 14 hours.It is suggested that such seeds treatment is to be recommanded to areas of severe incidence of these diseases.
Gu Benkang, Li Jingyi.
An Improved Technique for Inoculating Cotton Seed with Fusarium and Verticillium Wilt Pathogens and a Method of Disinfection[J]. Acta Phytopathologica Sinica, 1983, 13(1): 23-30