Application of ISEM method in the diagnosis of three potyviruses-Soybean mosaic virus (SMV),peanut mottle virus (PMV) and Cowpea aphid borne mosaic virus (CAMV) was carried out.Virus particles Were traped on the films of EM.grids which were coated with antiserum.Over 20-50 times of virus particles was trapped on homologous antiserum-coated grid than heterlogous or coating with normal serum.The sensitivity and specificity of ISEM depended on the concentration of antiserum coated on grids and the incubation times with the crude extracts from the diseased leaf.Results shoucd that the optimal dilution of antiserum were Ⅰ:Ⅰ,000.A short incubation time with virus suspenion trapped virus particles while prolonged incubation time increased trapping activity,but no increase happened after 10-20 hours incubation.
Chen Yong-xuan, Shi Zhi-xin, Xue Bao-di.
Diagnosis of Three Potyviruses Using Immunosorbent Electron Microscopy[J]. Acta Phytopathologica Sinica, 1987, 17(1): 34-39