金氏B (KB)培养基是荧光假单胞菌检测中应用最广泛的培养基,但是,其中有些有助荧光色素产生的成分在中国不易买到。我们将用国产蛋白胨、英国产胰蛋白胨和国产琼脂制备的金氏B培养基与用Difco的(月示)蛋白胨3号利琼脂制备的金氏B培养基进行了比较。6株荧光假单胞菌的试验结果表明,二者平板上生长情况没有明显差异,但是用国产蛋白胨(上海生物化学试剂)利琼脂粉(上海生化试剂)制备的金氏B平板上有90-100%的菌落没有荧光出现,而用英国产胰蛋白胨(oxoid)(20g/1)和国产琼脂(深圳)(15g/1)制备的金氏B平板上出现稳定而强烈的荧光,后者与用Difco试剂制备的金氏B培养基相比还能在非荧光菌株与荧光假单胞菌混合涂平板时稍微提高非荧光菌株的回收率。

King's medium B (KB) is the most widely used medium for the detection of fluorescent pseudomonads, but some of the recommended ingredients which couse this medium to enhance fluorescent pigment production are not readily available in China.KB made with locally available peptone, tryptone, and agar was compared with KB made with Proteose Peptone No.3 and Bacto Agar (Difco). The plating efficiency of six isolates of fluorescent pseudoraonads on KB made with local materials was not significantly different from KB made with Difco products. However, as many as 90% and 100% of the colonies failed to fluoresce on KB made with peptone or agar powder (Shanghai Biochemical Reagents), respectively.Consistent and intense fluorescent pigment production was obtained on KB made with Tryptone(Oxoid)(20 g/l) and agar strips (Shenzhen Agar Industry)(15 g/l). This latter medium promoted slightly greater recovery of non-fluorescent organisms when plated in combination with fluorescent pseudomonads as compared to KB made with Difco products.