Identification and phylogenetic analysis of Sclerotium hydrophilum from rice in Guangxi province
HU Chun-jin1,3, WEI Yuan-wen2,3, HUANG Si-liang1, LI Yang-rui2
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1. Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 2. Guangxi Crop Genetic Improvement and Biotechnology Lab, Nanning 530007, China; 3. Agricultural College of Guangxi University, Nanning 530005, China
A fungus was isolated from diseased rice in Guangxi,and the pathogenicity test indicated that its isolates could infect rice,corn and soybean under certain conditions.Based on microscopic morphology,other biological characteristics and ribosomal DNA-ITS sequence,the isolates obtained were identified as Sclerotium hydrophilum Sacc.,had an optimum temperature for hyphal growth at 30℃ and an optimal pH 6-9 in the culture media,and were prototrophic for thiamine.Its vegetative mycelia were similar to that of Rhizoctonia spp.The sclerotia,round and oval in shape,formed on the surface of PDA;the size of individual sclerotium was 386.4 μm×420.7 μm.Its sequence of ribosomal DNA-ITS was similar to that of S.hydrophilum(GenBank accession number:DQ875597) with 99.5% identity,and showed high homology with the strains of Rhizoctonia spp.,which was further demonstrated by the restriction analysis of an amplified fragment from 28S ribosomal DNA of these species.One specific primer pair,PS-1/PS-2,was designed from the sequence of ribosomal DNA-ITS of S.hydrophilum based on the conserved sequence determined by alignment with those of Rhizoctonia spp.By using this primer pair PS-1/PS-2,a band of 550 bp was amplified from S.hydrophilum but not from Rhizoctonia spp.
HU Chun-jin, WEI Yuan-wen, HUANG Si-liang, LI Yang-rui.
Identification and phylogenetic analysis of Sclerotium hydrophilum from rice in Guangxi province[J]. Acta Phytopathologica Sinica, 2008, 38(1): 17-23