同步检测为害百合种球3种检疫性病毒的多重RT-PCR方法

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植物病理学报 ›› 2009, Vol. 39 ›› Issue (3) : 321-324.

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研究简报

同步检测为害百合种球3种检疫性病毒的多重RT-PCR方法

杨翠云¹, 张丽莉², 于翠¹, 戚龙君¹

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Simultaneous detection of three quarantine viruses infecting lily bulbs by multiplex RT-PCR

YANG Cui-yun¹, ZHANG Li-li², YU Cui¹, QI Long-jun¹

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摘要

The multiplex RT-PCR approach was developed for simultaneous detections of Arabis mosaic virus(ArMV),Strawberry latent ringspot virus(SLRSV)and Lily symptomless virus(LSV)from the imported lily bulbs.The results indicated that good specificity and sensitivity for simultaneous detection were obtained.The ultimate of RNA detection with three viruses mixture was 305 pg.The ultimate of RNA detection with ArMV,SLRSV and LSV were 156.0,13.4 pg and 1.12 ng respectively.This approach has potential to be used in quarantine of imports and exports.

Abstract

导出引用

杨翠云, 张丽莉, 于翠, 戚龙君. 同步检测为害百合种球3种检疫性病毒的多重RT-PCR方法[J]. 植物病理学报, 2009, 39(3): 321-324

YANG Cui-yun, ZHANG Li-li, YU Cui, QI Long-jun. Simultaneous detection of three quarantine viruses infecting lily bulbs by multiplex RT-PCR[J]. Acta Phytopathologica Sinica, 2009, 39(3): 321-324